Synthetic Lysophospholipid Characterization

Synthetic lysophospholipids were purchased from Avanti Polar Lipids (Alabaster, AL, USA). FAs and 2-p-toluidinonaphthalene-6-sulphonate (TNS) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Digestive enzymes (porcine pepsin and trypsin, bovine α-chymotrypsin) as well as model protein substrates (bovine α-casein and cytochrome c) were purchased from Sigma-Aldrich. The recombinant Gly m 4 was overexpressed in E. coli and purified as described previously [15 (link)].

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Finkina E.I., Melnikova D.N., Bogdanov I.V., Ignatova A.A, & Ovchinnikova T.V. (2021). Do Lipids Influence Gastrointestinal Processing: A Case Study of Major Soybean Allergen Gly m 4. Membranes, 11(10), 754.

Publication 2021

Synthetic lysophospholipids 2-p-toluidinonaphthalene-6-sulphonate porcine pepsin bovine α-chymotrypsin bovine α-casein cytochrome c

Alabaster Bovine Casein Chymotrypsin Cytochrome c Digestive E coli Enzymes Lipids Lysophospholipids Pepsin Porcine Protein Sulphonate Trypsin

Corresponding Organization : Institute of Bioorganic Chemistry

Other organizations : Sechenov University

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Variable analysis

independent variables

Synthetic lysophospholipids
Fatty acids (FAs)
2-p-toluidinonaphthalene-6-sulphonate (TNS)
Digestive enzymes (porcine pepsin and trypsin, bovine α-chymotrypsin)
Model protein substrates (bovine α-casein and cytochrome c)
Recombinant Gly m 4 overexpressed in E. coli

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

Positive controls: None specified
Negative controls: None specified

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