Synthetic TGN liposomes were prepared using a mixture of lipids that approximates the lipid composition of the TGN (Klemm et al., 2009 (link)). Lipids were combined, vacuum dried, rehydrated in HK buffer (20 mM Hepes, pH 7.4, and 150 mM KOAc), and extruded through 100-nm filters (Whatman) to generate liposomes. See Table S3 for the composition of TGN and phosphatidylcholine liposomes.
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Synthetic TGN Liposome Preparation
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Corresponding Organization : Cornell University
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Variable analysis
independent variables
- Lipid composition of TGN liposomes
- Formation of liposomes
- HK buffer (20 mM Hepes, pH 7.4, and 150 mM KOAc)
- Extrusion through 100-nm filters (Whatman)
- Phosphatidylcholine liposomes as a control (see Table S3)
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