Comparative Structural Analysis of Yersinia Ail Proteins

Y. pestis and Y. pseudotuberculosis Ail sequences in GenBank (National Health Institute, NIH) as of October 2021 were analyzed with ClustalW for multiple sequence alignment. The deep learning-based modeling method, RoseTTAFold, was used to generate Ail structural models that were superimposed and compared with UCSF Chimera plug-in, MatchMaker (69 (link), 70 (link)).

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Kolodziejek A.M., Bearden S.W., Maes S., Montenieri J.M., Gage K.L., Hovde C.J, & Minnich S.A. (2023). Yersinia pestis Δail Mutants Are Not Susceptible to Human Complement Bactericidal Activity in the Flea. Applied and Environmental Microbiology, 89(2), e01244-22.

Publication 2023

Chimera Pseudotuberculosis Sequence alignment Y pestis

Corresponding Organization : University of Idaho

Other organizations : Centers for Disease Control and Prevention

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Variable analysis

independent variables

Y. pestis
Y. pseudotuberculosis

dependent variables

Ail sequences in GenBank (National Health Institute, NIH) as of October 2021
Ail structural models generated using RoseTTAFold
Superimposed and compared Ail structural models using UCSF Chimera plug-in, MatchMaker

control variables

Control variables not explicitly mentioned.

controls

Positive control: Not specified.
Negative control: Not specified.

Annotations

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