Rubia cordifolia root material was purchased from the Wandong Bridge Chinese herbal medicine market in Nanming District, Guiyang City, Guizhou Province, and was positively identified by Associate Professor Long Qingde of Guizhou University of Traditional Chinese Medicine. The root material was boiled twice, and the decoctions from the two boilings were combined. The combined decoctions were filtered and concentrated to 1 g/mL, which is equivalent to raw material, and stored at 4°C for later use. This experiment has been approved by the Ethics Committee of Qionghai People's Hospital (Project No.: QH-20201120-0012).
Thirty-two healthy adult SD rats were randomly divided into the sham group (sham), the model group (model), the madder group (madder), and the diltiazem group (Dil). Seven days before surgery, rats in the model group and the sham group were injected with 1 mL normal saline every day, rats in the madder group were injected with madder extract (0.5 mg/kg) every day, and rats in the Dil group were injected with diltiazem (0.2 mg/kg) every day. I/R modeling was performed in the model group, the madder group, and the Dil group.
For the performance of the myocardial ischemia-reperfusion model [10 (link)], rats were anesthetized with pentobarbital sodium and immobilized on the operating table. Endotracheal intubation was performed on the rats, and a positive pressure ventilator was implanted to assist respiration. Left 3–4 rib space thoracotomy was done to break the pericardium to expose the heart. The left anterior descending coronary artery was ligated using a surgical lead. After ligation of the anterior descending branch for 30 min, the ligation line was relaxed, and reperfusion was performed for 1 h. The sham operation group was not subjected to ligation of the anterior descending branch, but the other steps were the same. Arterial flow and myocardial systolic rate were measured after operation.
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