ECAR and OCR were measured in real-time with Glycolysis Stress Test Kit and Mito Stress Test Kit, respectively, using the Seahorse XFe96 Analyser (Seahorse Biosciences, USA) according to the previous study [31 (link)]. Data were normalized by cell numbers that was measured by the YO-PRO®-1 Assay (Thermo Fisher Scientific).
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