Fresh plant samples were harvested on the 5th and 10th days of treatment, and then the fresh leaves were cut into small pieces for sample preparation to study the stomatal structure. Afterwards, the leaves were fixed in the first fixative solution i.e., glutaraldehyde solution, for 2–3 h with pH 7.4. After the fixation step, the next step that followed was dehydration, in which the samples were dehydrated with an ethanol series ranging from 95% ethanol to 50% ethanol. The next process for visualizing the structure of stomata was performed using scanning electron microscope (model: EVO-18 Research, Carl Zeiss, United States of America) [36 (link)].
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