Responder peripheral blood lymphocytes (PBL) were plated in triplicate in 96-well flat-bottom plates (Costar, Cambridge, MA) at a final concentration of 4 × 105 cells/well in RPMI 1640 supplemented with 6% fetal pig serum and were stimulated by an equal number of irradiated (2500 cGy) stimulator PBL as previously described (14 (link)). For tests of mixed lymphocyte reaction (MLR) in the presence of cyclosporine, the drug was added to the medium at a final concentration of 0, 5, 10, 20 and 40 ng/mL.
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Mixed Lymphocyte Reaction with Cyclosporine
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Other organizations : Harvard University
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Variable analysis
independent variables
- Concentration of cyclosporine (0, 5, 10, 20, 40 ng/mL)
- Lymphocyte activation in mixed lymphocyte reaction (MLR)
- Responder peripheral blood lymphocytes (PBL) concentration (4 × 10^5 cells/well)
- Stimulator PBL concentration (equal to responder PBL)
- Stimulator PBL irradiation (2500 cGy)
- Culture medium (RPMI 1640 supplemented with 6% fetal pig serum)
- Positive control: MLR without cyclosporine (0 ng/mL)
- Negative control: Not explicitly mentioned
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