Methionine Supplementation in Fish Meal-Soy Protein Diet

The basic diet (110 g/kg fish meal and 400 g/kg soy protein concentrate) was based on our previous data [15 (link),16 (link)]. Different levels of methionine (0, 2, 4, 6, 8, or 10 g/kg) were supplemented in the basic diet based on the rule of equal nitrogen and our previous studies [14 (link),16 (link)], showed in Table 6, Table 7 and Table 8.
Proximate analysis (moisture, crude protein, crude lipid, ash, and gross energy) of experimental feed and M. albus was performed based on our previous papers [53 (link)]. Amino acids were analyzed by an automatic amino acid analyzer (Agilent-1100, Agilent Technologies Co., Ltd., Santa Clara, CA, USA) based on Wijerath’s method [54 (link)], and fatty acids were analyzed by GC-MS (Agilent 7890B-5977A, Agilent Technologies Co., Ltd., Santa Clara, CA, USA) based on Jin’s method [55 (link)], the results are shown in Table 2 and Table 3.