A total of 200 chickens were obtained from the Poultry Institute of the Chinese Academy of Agricultural Sciences (Yangzhou, China), including 100 Dagu chickens and 100 Wenchang chickens. The two chicken breeds were both from gene pool preservation populations. After hatching, all chicks were housed in an environmentally controlled room until 7 days of age and then transferred to sterilized isolation ventilated cages (IVCs) (IPQ-type 3 negative pressure isolator). The chicks were randomly divided into two groups: noninfected (25 individuals) and Salmonella typhimurium (ST)-infected (75 individuals) in each breed. These birds of the ST-infected group were randomly selected for Salmonella infection at 14 and 28 days of age. After Salmonella infection, the birds of each group were assigned to one IVC. The chicks received ad libitum access to feed and water throughout the experiment.
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