Methods for urine and plasma analysis were conducted as previously described30 (link). Urinary aldosterone was measured using an aldosterone I125 radioimmunoassay (Tecan, Morrisville, NC), and standardized to urinary creatinine (Jaffe’ colorimetric assay, Cayman Chemicals, Ann Arbor, MI) in order to account for differences in GFR and urine volume. Plasma renin concentration was determined by RIA (Diasorin, Stillwater, MN). Blood chemistries were determined from retro-orbital sinus sampling using heparinized capillary tubes for analysis with an iStat hand held analyzer (EC8+ cartridge, Heska, Fort Collins, CO). Urinary Na+ and K+ were measured by flame photometry (IL943 Automatic Flame Photometer, Instrumentation Laboratory, Inc.).
Protocol detail
Urine and Plasma Analysis Methods
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Aldosterone
Assay
Blood chemistries
Capillary
Cayman
Colorimetric
Creatinine
Held
I125
Photometry
Plasma
Renin
Sinus
Urinary
Corresponding Organization :
Other organizations : University of Virginia, Boston Children's Hospital, Harvard University
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Variable analysis
independent variables
- None explicitly mentioned
- Urinary aldosterone levels
- Plasma renin concentration
- Blood chemistries (including sodium and potassium)
- Urinary creatinine levels (used to standardize urinary aldosterone measurements)
- Urine volume (used to standardize urinary aldosterone measurements)
- No positive or negative controls were explicitly mentioned in the provided information.
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