The following fly stocks were used: Oregon R, w1118, Ubx1/TM6B, Tb, Sb, Dfd-lacZ and UAS-Ubx (kindly provided by L. S. Shashidara, IISER, Pune, India), Ubx6.28/TM6B, Tb, Sb, Dfd-lacZ, abdAMX1/TM3, Sb, Kr-Gal4, UAS-GFP (Sánchez-Herrero et al., 1985 (link)), Hb9-Gal4/TM3, Sb, ftz-lacZ (Broihier et al., 2002 (link)), 24B-Gal4, UAS-mCherry/TM6B (kindly provided by S. Merabet, IGFL, Lyon, France), Mef2-Gal4, UAS-CD4::td-tom.FP/TM6B (kindly provided by O. Vef, University of Mainz, Germany), Act5C-Gal4/CyO, Wnt4EMS23, bw/CyO, hb-lacZ, arm4/FM7, grh-lacZ, arm8/FM7c,Dfd-GMR-nvYFP, UAS-mCherry RNAi, UAS-Ubx RNAi, UAS-arm RNAi, UAS-sgg.B (UAS-GSK3), UAS-dTCFΔN (UAS-dTCF.DN), UAS-CD4::tdGFP and UAS-CD8::GFP (all from Bloomington Stock Center, Indiana, USA). arm8/X or arm8/X;; Ubx1/+ animals were identified by the anti-Sex lethal signal.
The UAS-Ubx RNAi insertion on the second chromosome (attP40) was generated using the shUbx RNAi (HMS01403) construct in pValium20 (Ni et al., 2011 (link)) (kindly provided by the TRiP consortium, Harvard, USA). All experiments were performed at 25°C except for the RNAi and dominant-negative experiments, which were incubated at 29°C.
The UAS-Ubx RNAi insertion on the second chromosome (attP40) was generated using the shUbx RNAi (HMS01403) construct in pValium20 (Ni et al., 2011 (link)) (kindly provided by the TRiP consortium, Harvard, USA). All experiments were performed at 25°C except for the RNAi and dominant-negative experiments, which were incubated at 29°C.
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