The sequences of the oligonucleotides were listed in Supplementary Table S1 . HPLC-purified fluorescent labeled primers, biotinylated extension oligonucleotides, and splint oligonucleotides were purchased from Integrated DNA Technologies (Coralville, IA, USA). Single-stranded oligonucleotides with ICL precursors, 39mer 20-bp ICL that contains two franking uracil bases around the crosslinked base (39+20(6)-ICL), and 39mer 6-bp ICL (39+6-ICL) were synthesized and purified as previously reported (37 (link)). The identity of the ICLs was confirmed by LC–MS (Supplementary Figure S1A ).
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Synthesis and Characterization of DNA Interstrand Crosslinks
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Corresponding Organization : Washington University in St. Louis
Other organizations : Institute for Basic Science, Stony Brook University, Columbia University, Ulsan National Institute of Science and Technology
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Variable analysis
independent variables
- Sequences of the oligonucleotides
- HPLC-purified fluorescent labeled primers
- Biotinylated extension oligonucleotides
- Splint oligonucleotides
- Single-stranded oligonucleotides with ICL precursors
- 39mer 20-bp ICL that contains two franking uracil bases around the crosslinked base (39+20(6)-ICL)
- 39mer 6-bp ICL (39+6-ICL)
- Not explicitly mentioned
- Not explicitly mentioned
- Positive control: Not specified
- Negative control: Not specified
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