Screening Protocol for T2D Subjects

This manuscript builds on data from two different studies, both including subjects with T2D (25 (link)). Exclusion criteria were pregnancy, smoking, contraindication to increased levels of physical activity (26 (link)), insulin dependence, and evidence of thyroid, liver, lung, heart, or kidney disease. All subjects underwent a screening consisting of a medical interview and examination, an oral glucose tolerance test (OGTT), a walking VO₂peak test with indirect calorimetry (Cosmed K4B2, Rome, Italy) and a familiarization VO₂max test performed on a treadmill (Katana Sport, Lode, Groningen, the Netherlands) with indirect calorimetry (Cosmed Quark, Rome, Italy) as previously described (22 (link), 27 (link)). Written and informed consent was obtained from all research participants before any investigations were performed and the studies were approved by the Ethical Committee of the Capital Region of Denmark (H-6-2014-043 and H-1-2013-116) and registered at www.ClinicalTrials.gov (NCT02320526) and (NCT02089477).