Glioma Stem Cell and Xenograft Model

The human glioma stem/progenitor cell line SU3 and nude mice expressing EGFP (NC-CB57/6J-EGFP) were prepared by our laboratory [4 (link), 5 (link)]. The remaining reagents were purchased from companies as follows: rat C6 glioma cell line (Shanghai Institutes for Biological Sciences), RFP transgenic kit (Genechem, Shanghai), γ-secretase inhibitors DAPT (GSI-IX) (Selleck), Dulbecco's Modified Eagle's Medium (Gibco), fetal bovine serum (Hyclone), Caspase—Glo 3/7 assay kit (Promega), Trizol solution (Invitrogen), reverse transcription kit (Fermentas), ECL chemiluminescence reagent, trypan blue, GAPDH antibody (Biyuntian, Shanghai), Notch-1, NICD, Bcl-2 and pAKT antibodies (Cell Signal), 2', 3'-cyclic nucleotide 3' phosphodiesterase (CNP) monoclonal antibody (Abcam), qPCR apparatus and SYBR Green qPCR Mix (BioRad), apoptosis kit (BD), flow cytometry instrument (Beckman), inverted fluorescence microscope (Carl Zeiss), linear accelerator (Siemens Primus), and in vivo fluorescence imaging system (Maestro EX, CRi).

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Shen Y., Chen H., Zhang J., Chen Y., Wang M., Ma J., Hong L., Liu N., Fan Q., Lu X., Tian Y., Wang A., Dong J., Lan Q, & Huang Q. (2015). Increased Notch Signaling Enhances Radioresistance of Malignant Stromal Cells Induced by Glioma Stem/ Progenitor Cells. PLoS ONE, 10(11), e0142594.

Publication 2015

DAPT (GSI-IX) Dulbecco's Modified Eagle's Medium Caspase—Glo 3/7 assay kit Trizol solution reverse transcription kit ECL chemiluminescence reagent SYBR Green qPCR Mix flow cytometry instrument inverted fluorescence microscope

Antibodies Antibody Apoptosis Assay Bcl 2 Caspase 7 Cell Cell line Chemiluminescence Cyclic nucleotide Dapt Fetal bovine serum Flow cytometry Fluorescence microscope Gapdh Glioma Human Inhibitors Linear accelerator Monoclonal antibody Nude mice Phosphodiesterase Progenitor cell Promega Reverse transcription Secretase Stem Sybr green Transgenic Trizol Trypan blue

Corresponding Organization : Jiangsu Province Hospital

Top 5 similar protocols

Variable analysis

independent variables

Treatment with γ-secretase inhibitors DAPT (GSI-IX)

dependent variables

Apoptosis of glioma stem/progenitor cells
Expression of Notch-1, NICD, Bcl-2, and pAKT proteins
Expression of 2', 3'-cyclic nucleotide 3' phosphodiesterase (CNP) mRNA

control variables

Human glioma stem/progenitor cell line SU3
Nude mice expressing EGFP (NC-CB57/6J-EGFP)
Rat C6 glioma cell line
RFP transgenic kit
Dulbecco's Modified Eagle's Medium
Fetal bovine serum
Caspase-Glo 3/7 assay kit
Trizol solution
Reverse transcription kit
ECL chemiluminescence reagent
Trypan blue
GAPDH antibody
Notch-1, NICD, Bcl-2 and pAKT antibodies
2', 3'-cyclic nucleotide 3' phosphodiesterase (CNP) monoclonal antibody
QPCR apparatus and SYBR Green qPCR Mix
Apoptosis kit
Flow cytometry instrument
Inverted fluorescence microscope
Linear accelerator
In vivo fluorescence imaging system

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