Silencing HIF1A Expression with Lentiviral shRNA
Corresponding Organization : University of Naples Federico II
Other organizations : Ceinge Biotecnologie Avanzate (Italy), Istituto Giannina Gaslini
Variable analysis
- ShRNA targeting HIF1A: V2LHS_132152 (shHIF1A#A) and V2LHS_236x718 (shHIF1A#B)
- Non-silencing pGIPZ lentiviral shRNAmir as the control (RHS4346)
- HIF1A expression
- Lentivirus particles production and cell infection procedure as previously described [12]
- Puromycin selection to obtain 100% GFP-positive cells
- Positive control: shRNAs targeting HIF1A (shHIF1A#A and shHIF1A#B)
- Negative control: Non-silencing pGIPZ lentiviral shRNAmir (RHS4346)
Annotations
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