The allotetraploid cotton cultivar, Xuzhou 142, was grown in Shaanxi Normal University under controlled conditions (He et al., 2017 (link)). A total of 30 ovules were used for each phytohormone and were performed in triplicate. Cotton ovules were collected at one DPA, sterilized with sodium hypochlorite (NaClO, 10%), and cultivated as previously reported (Shi et al., 2006 (link)). Five μM 1-Naphthylacetic acid (NAA, Sigma, Germany) and 1 μM GA3 (Sigma, Germany) were added to the culture medium. The ovules treated with phytohormones were used to perform RNA-seq, while the data was conserved in our lab (He et al., 2019 ).
To illustrate the spatial and temporal expression patterns of GhHXKs, the transcriptomes of various tissues (stamen, anther, seed, fiber, ovule, petal, calycle, torus, leaf, stem, root, cotyledon, stigma, and pistil) and a successive fiber developmental stages (0, 5, 10, 15, 20, 25, 30, and 35 DPA) were downloaded from NCBI (accession NO.PRJNA680449 ) (Ma et al., 2021 (link)). The expression data were normalized and visualized using Omicshare tools.13
To illustrate the spatial and temporal expression patterns of GhHXKs, the transcriptomes of various tissues (stamen, anther, seed, fiber, ovule, petal, calycle, torus, leaf, stem, root, cotyledon, stigma, and pistil) and a successive fiber developmental stages (0, 5, 10, 15, 20, 25, 30, and 35 DPA) were downloaded from NCBI (accession NO.
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