Western Blot Analysis of ALV-J Envelope

Western blotting was performed following our previously described method with ALV-J envelope protein specific mouse anti-monoclonal antibody JE9 (kindly provided by Dr. Aijian Qin, Yangzhou University, Yangzhou, China) and rabbit anti-β-actin antibody [22 (link)]. IRDye 700DX-conjugated anti-rabbit IgG and IRDye 800-conjugated anti-mouse IgG (Rockland Immunochemicals, Limerick, PA, USA) were used as the secondary antibody. Membranes were visualized and analyzed with an Odyssey infrared imaging system (LI-COR Biosciences, Lincoln, NE, USA).

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Dai M., Feng M., Xie T., Li Y., Ruan Z., Shi M., Liao M, & Zhang X. (2017). ALV-J infection induces chicken monocyte death accompanied with the production of IL-1β and IL-18. Oncotarget, 8(59), 99889-99900.

Publication 2017

IRDye 700DX-conjugated anti-rabbit IgG IRDye 800-conjugated anti-mouse IgG Odyssey infrared imaging system

Actin Anti antibody Anti igg Antibody Envelope protein Irdye 800 Membranes Monoclonal antibody Mouse Rabbit

Corresponding Organization :

Other organizations : South China Agricultural University, Virginia–Maryland College of Veterinary Medicine, University of Maryland, College Park

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Variable analysis

independent variables

ALV-J envelope protein specific mouse anti-monoclonal antibody JE9

dependent variables

Protein expression levels

control variables

Rabbit anti-β-actin antibody

controls

Positive control: rabbit anti-β-actin antibody
Negative control: Not explicitly mentioned

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