Establishing In Vitro Models for Cancer Research

Human head and neck cancer cell lines (CAL27 (CRL2095), Detroit 562 (CCL-138)), murine lung (LL/2 (CRL-1642)) and murine melanoma (B16 (CRL-6475)) were obtained from American Type Culture Collection. Murine colorectal (MC38 (ENH204-FP)) cancer cell lines were obtained from Kerafast. Murine head and neck MOC1 and MOC2 were generously provided by Dr. R. Uppaluri. CAL27 PIK3CA H1047R expressing cells and 4MOSC1 cells were generated in Dr. Gutkind’s laboratory and previously characterized^{42 (link),55 (link)}. CAL27, Detroit 562, LL/2, and B16 cells were cultured in DMEM (Gibco) supplemented with 10% FBS (Omega Scientific). MC38 cells were cultured in DMEM supplemented with 10% FBS, 1 mM sodium pyruvate (Gibco), 1% non-essential amino acids (Gibco), and 10 mM HEPES (Gibco). MOC1 and MOC2 cells were cultured in IMDM (Lonza)/F-12K nutrient mixture at a 2:1 mixture with 5% FBS, 5 ng/ml EGF (EMD Millipore), 400 ng/ml hydrocortisone (Sigma), and 5 mg/ml insulin (Sigma). On receipt, each cell line was expanded, cryopreserved as low passage stocks, and routinely tested for mycoplasma. Cisplatin (Enzo Biosciences), paclitaxel (Sigma), and MMAE (Concortis) were reconstituted in DMSO. Clinical-grade CDX3379 was provided by Celldex. Clinical grade cetuximab (Erbitux, Lilly Medical) was obtained from UCSD Moores Cancer Center Pharmacy.