R28 cells with a density of 5 × 103 cells/well were inoculated in a 96-well cell culture plate and cultivated overnight at 37°C and 5% CO2. After the treatment with drugs for 24 h, 10 µl CCK-8 solution (Vazyme, Cat. No A311-01/02) was added to each well and the cells were cultivated for another 4 h at 37°C in 5% CO2. Cell viability evaluation was conducted by measuring absorbance at 450 nm with the use of a Varioskan™ LUX Multi-function microplate reader (Thermo Fisher Scientific, Inc.) [22 (link)].
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