Bradykinin-Induced Plasma Coagulation Assay

Penicillin G sodium salt (1,550 U/mg) and other beta-lactams were from Solarbio Life Sciences (Beijing, China) and Yuanye Biotechnology Ltd. (Shanghai, China), respectively. Icatibant was from MedChemExpress (Monmouth Junction, NJ, USA). S-2302™ was from Chromogenix (Milano, Italy). BK ELISA kit was from DS Pharma Biomedical Co., Ltd. (Osaka, Japan). Kaolin, dextran sulfate sodium salt (DS; Mr ~ 500,000), propranolol, triprolidine, SB290157 and CV3988 were from Sigma-Aldrich (Darmstadt, German). PMX53 and pertussis toxin (PTX) were from GL Biochem Ltd. (Shanghai, China) and Enzo Life Sciences (Farmingdale, NY, USA), respectively. Citrate-anticoagulant standard human plasma and FXII-depleted plasma were from Boatman Biotech Co., Ltd. (Shanghai, China). Antibodies for human FXII and transferrin were from GeneTex Inc. (San Antonio, TX, USA). Shrimp tropomyosin (ST) and its IgE monoclonal antibody (mAb) were prepared as we previously described^{23 (link)}. Human plasma was obtained from eight healthy volunteers.

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Gao Y., Han Y., Zhang X., Fei Q., Qi R., Hou R., Cai R., Peng C, & Qi Y. (2020). Penicillin causes non-allergic anaphylaxis by activating the contact system. Scientific Reports, 10, 14160.

Publication 2020

Penicillin G sodium salt Icatibant S-2302™ propranolol triprolidine SB290157 CV3988 PMX53

Antibodies Anticoagulant Beta lactams Citrate Cv3988 Dextran sulfate sodium Elisa Healthy volunteers Human Icatibant Kaolin Monoclonal antibody Penicillin g sodium salt Pertussis toxin Plasma Propranolol Salt Sb290157 Transferrin Triprolidine Tropomyosin

Corresponding Organization : Chengdu University of Traditional Chinese Medicine

Other organizations : Chinese Academy of Medical Sciences & Peking Union Medical College

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Variable analysis

independent variables

Penicillin G sodium salt (1,550 U/mg)
Other beta-lactams
Icatibant
S-2302™
Kaolin
Dextran sulfate sodium salt (DS; Mr ~ 500,000)
Propranolol
Triprolidine
SB290157
CV3988
PMX53
Pertussis toxin (PTX)
Shrimp tropomyosin (ST) and its IgE monoclonal antibody (mAb)

dependent variables

Measured outcomes not explicitly mentioned

control variables

Citrate-anticoagulant standard human plasma
FXII-depleted plasma
Antibodies for human FXII and transferrin
Human plasma obtained from eight healthy volunteers

positive controls

Citrate-anticoagulant standard human plasma

negative controls

FXII-depleted plasma

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