Synthetic Biosynthetic Gene Constructs

Full-length P. somniferum PsTyDC1 native coding sequence was synthesized by Integrated DNA Technologies (IDT, Coralville, IA, USA). Codon optimization of PsONCS3 and TfNCS nucleotide sequences^{60 (link)} for expression in E. coli was assisted by Codon Optimization OnLine (COOL)^{61 (link)}, resulting in the coding sequences shown in Supplementary Table 9, and the selected sequences were synthesized by IDT. The native sequence of full-length P. somniferum NMCH isoform 1 (PsNMCH-I1) was also synthesized by IDT.
Native coding sequences of full-length PsPDC1, full-length Ps2HCLL, and N-terminal truncated PsPDC2 were synthesized and cloned into pBAD-DEST49 (LifeSensors Inc., Malvern, PA, USA) via the Gateway cloning system by GeneArt (Invitrogen, Waltham, Massachusetts, USA). Native coding sequences of full-length EcNMCH, AtATR2, and P. somniferum CPR-like (PsCPR-L) were synthesized and subcloned into the pMA vector by GeneArt (Invitrogen). Native coding sequences of full-length PsTyDC6 and N-terminal truncated PsPDC1-IX1 were synthesized and cloned into pTYB21 (NEB) by GenScript (Piscataway, NJ, USA).