With reference to the dosages used in other studies24 (link),40 (link), CDPPB (Tocris) was dissolved in DMSO to a concentration of 10 mg/ml, and mixed with PBS containing 10% Tween-80 in a 1:9 ratio for intraperitoneal injection. PBS containing 10% Tween-80 was given as vehicle. Mice were given a single intraperitoneal injection at 10 mg/kg/day from P16 to 22. The whole little was weaned at P35.
For rescue experiments performed in adult mice, only males were employed. CDPPB (10 mg/kg) was injected 30 min before the open field test and three-chamber test. Cortices were collected within 10 min after the open field test. Protein samples were prepared in IP buffer supplemented with inhibitors including sodium orthovanadate. Samples were probed with antibodies against phospho-ERK (Cell Signaling, cat. 9101), total ERK (Cell Signaling, cat. 9102), phospho-JNK (Cell Signaling, cat. 9251), and total JNK (Cell Signaling, cat. 9252).
For rescue experiments performed in adult mice, only males were employed. CDPPB (10 mg/kg) was injected 30 min before the open field test and three-chamber test. Cortices were collected within 10 min after the open field test. Protein samples were prepared in IP buffer supplemented with inhibitors including sodium orthovanadate. Samples were probed with antibodies against phospho-ERK (Cell Signaling, cat. 9101), total ERK (Cell Signaling, cat. 9102), phospho-JNK (Cell Signaling, cat. 9251), and total JNK (Cell Signaling, cat. 9252).
Free full text: Click here
