miR-negative control (NC) mimic, miR-30e-5p mimic, pcDNA3.1, and pcDNA3.1-TLR4 were purchased from Shanghai GenePharma Co., Ltd. Rat is an ideal pathophysiological disease model that is widely used (23 (link),24 (link)). The ICH model in rats was established by IC injection of collagenase type IV (0.23 U in 1 µl sterile saline; Beijing Solarbio Science & Technology Co., Ltd.) into the right striatum, as previously described (25 (link)). For the rats in the ICH group (n=11), miR-NC mimic + pcDNA3.1 was injected into the right lateral ventricle of rats 3 days prior to the establishment of ICH model.
Rats in the sham group (n=11) were injected with 1 µl sterile saline. For rats in the remaining groups (ICH + miR-30e-5p mimic + pcDNA3.1 and ICH + miR-30e-5p mimic + pcDNA3.1-TLR4; both n=11), miR-30e-5p mimic + pcDNA3.1 or miR-30e-5p mimic + pcDNA3.1-TLR4 was injected into the right lateral ventricle of rats 3 days prior to establishment of ICH model (26 (link)).
Rats in the sham group (n=11) were injected with 1 µl sterile saline. For rats in the remaining groups (ICH + miR-30e-5p mimic + pcDNA3.1 and ICH + miR-30e-5p mimic + pcDNA3.1-TLR4; both n=11), miR-30e-5p mimic + pcDNA3.1 or miR-30e-5p mimic + pcDNA3.1-TLR4 was injected into the right lateral ventricle of rats 3 days prior to establishment of ICH model (26 (link)).
