Quantifying Corneal Bacterial Burden

Corneas from siSTING versus siNC-treated BALB/c mice (at 5 days p.i.) or from cGAMP versus control-treated C57BL/6 mice (at 5 days p.i.) were pooled (n = 5/group/time). The number of viable bacteria was calculated as described before (30 (link)). Briefly, individual corneas were homogenized, diluted in a series, and seeded on Pseudomonas isolation agar (BD Difco Laboratories) in triplicate. Results are reported as log10 CFU per cornea ± SEM.

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Chen K., Fu Q., Liang S., Liu Y., Qu W., Wu Y., Wu X., Wei L., Wang Y., Xiong Y., Wang W, & Wu M. (2018). Stimulator of Interferon Genes Promotes Host Resistance Against Pseudomonas aeruginosa Keratitis. Frontiers in Immunology, 9, 1225.

Publication 2018

Pseudomonas isolation agar

Agar Bacteria Balb c mice C57bl mice Cgamp Cornea Isolation Pseudomonas Sinc

Corresponding Organization : Fifth Affiliated Hospital of Sun Yat-sen University

Other organizations : Third Affiliated Hospital of Sun Yat-sen University, Guangzhou University of Chinese Medicine

Top 5 similar protocols

Variable analysis

independent variables

SiSTING treatment of BALB/c mice
SiNC treatment of BALB/c mice
CGAMP treatment of C57BL/6 mice
Control treatment of C57BL/6 mice

dependent variables

Number of viable bacteria in corneas

control variables

Time point (5 days post-infection)
Mouse strain (BALB/c and C57BL/6)
Pooled cornea samples (n = 5 per group per time point)

controls

No positive control mentioned
No negative control mentioned

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