Endothelial Cell-Specific Genetic Manipulation

All mice used in this study were maintained on a C57BL/6 background and housed in a non-barrier facility. Wildtype C57BL/6 female mice were purchased from Jackson Laboratories. CDH5-CreER^T2 mice and animals with Rptor or Rictor floxed alleles were sourced and maintained as described previously^{4 ,71 (link)}. Tsc2 floxed mice were generated and provided by Kevin Ess (Vanderbilt University Medical Center^{76 (link)}. Animals were genotyped for Cre or floxed alleles of Rptor, Rictor, or Tsc2 alleles using the primers listed in Extended Data Table 1. To induce endothelial-specific deletion of Rptor, Rictor, or Tsc2, tamoxifen (T5648, Sigma) was reconstituted in sunflower seed oil (S5007, Sigma) and administered intraperitoneally (i.p.) for five consecutive days in 7 to 12-week-old mice at a final dose of 2 mg/mouse, as previously described⁴ . tamoxifen was administered beginning four days after intravenous tumor cell inoculation.