Immunohistochemical Analysis of Bladder Cancer

To obtain representative cores for the TMA construction, parallel sections stained by hematoxylin and eosin were used to identify a representative core position within the specimens. IHC staining was carried out according to the antibody manufacturers’ instructions. Tissue slides were incubated overnight at 4 °C with a human IL-1 beta/IL-1F2 (AF-201, R&D systems Inc., Minneapolis, MN, USA) and IL-1 RA/IL-1F3 (AF-280, R&D systems, Minneapolis, MN, USA) polyclonal goat immunoglobulin, respectively [12 (link),15 (link)], in dilutions 1:10 and 1:300, in real antibody diluent (DAKO, Glostrup, Denmark). After three more washing steps, a visualization was performed with Dako Liquid DAB-Substrat Chromogen System K3467 (DAKO, Glostrup, Denmark) and counterstaining with hematoxylin, as indicated by the manufacturer [27 (link)]. Two or more cores of every invasive BC and corresponding normal bladder tissue were integrated.

Free full text: Click here

Vukovic M., Chamlati J.M., Hennenlotter J., Todenhöfer T., Lütfrenk T., Jersinovic S., Tsaur I., Stenzl A, & Rausch S. (2024). Interleukin-1β/Interleukin (IL)-1-Receptor-Antagonist (IL1-RA) Axis in Invasive Bladder Cancer—An Exploratory Analysis of Clinical and Tumor Biological Significance. International Journal of Molecular Sciences, 25(4), 2447.

Publication 2024

AF-201 real antibody diluent

Corresponding Organization :

Other organizations : University of Tübingen, University of Montenegro

Top 5 similar protocols

Variable analysis

independent variables

Antibody used for IHC staining: human IL-1 beta/IL-1F2 (AF-201, R&D systems Inc., Minneapolis, MN, USA) and IL-1 RA/IL-1F3 (AF-280, R&D systems, Minneapolis, MN, USA) polyclonal goat immunoglobulin

dependent variables

IHC staining intensity

control variables

Dilution of antibodies (1:10 and 1:300)
Incubation time (overnight at 4 °C)
Washing steps (three times)
Visualization method (Dako Liquid DAB-Substrat Chromogen System K3467)
Counterstaining (hematoxylin)
Number of cores (two or more) from invasive BC and corresponding normal bladder tissue

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!