Western Blot Analysis of TGF-β Signaling

Whole cell lysates and western blotting were performed as described previously 9 (link). Antibodies against CD105 (ab169545, 1:1000), Smad2 (ab40855, 1:2000), pSmad3 (ab52903, 1:1000) were purchased from Abcam. An antibody against BMP9 (sc514211, 1:500) was purchased from Santa Cruz Biotechnology (USA). Antibodies against Smad1 (6944, 1:1000), pSmad1/5/8 (13820, 1:1000), and pSmad2 (3108, 1:1000) were purchased from Cell Signaling Technology. Antibodies against Smad3 (YM3417, 1:2000), GAPDH (YM3029, 1:20000), and β-tubulin (YM3030, 1:5000) were purchased from ImmunoWay Biotechnology.

Free full text: Click here

Wang X., Zong L., Wang W., Yang J, & Xiang Y. (2020). CD105 overexpression mediates drug-resistance in choriocarcinoma cells through BMP9/Smad pathway. Journal of Cancer, 11(2), 272-283.

Publication 2020

ab169545 ab40855 ab52903 sc514211 YM3029 YM3030

Antibodies Antibody Cell Gapdh Smad2 Smad3 Tubulin

Corresponding Organization :

Other organizations : Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College

Top 5 similar protocols

Variable analysis

independent variables

None explicitly mentioned

dependent variables

CD105 protein levels
Smad2 protein levels
PSmad3 protein levels
BMP9 protein levels
Smad1 protein levels
PSmad1/5/8 protein levels
PSmad2 protein levels
Smad3 protein levels
GAPDH protein levels
β-tubulin protein levels

control variables

None explicitly mentioned

controls

Positive control: None mentioned
Negative control: None mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!