Immunohistochemical Analysis of CSNK2A1 Protein

CSNK2A1 protein expression was determined via immunohistochemistry (IHC) analysis using paraffin-embedded liver sections. Anti-CSNK2A1 IHC staining was carried out using the LsAB Kit (DAKO, Glostrup, Denmark). All tissue sections were de-waxed, treated with Proteinase K enzyme, and the endogenous peroxidase activity was blocked by incubating with 3% hydrogen peroxide for 10 min. After washing with phosphate-buffered saline (PBS; pH 7.6) for 5 min, the slides were incubated with anti-CSNK2A1 antibodies (GTX84369; GeneTex, Hsinchu, Taiwan) for 30 min at 37 ℃, followed by rabbit anti-rat antibodies and a goat anti-rabbit HRP polymer for 15 min. The immunocomplexes were visualized using DAB solution (DAKO) for 5 min. Samples were washed with PBS (pH 7.6) in order to perform all the necessary steps 14 (link),15 (link),16 .