Genome Assembly of P. penicillatus

P. penicillatus CCMJ2836 genomic DNA was extracted from the mycelia using NuClean Plant Genomic DNA Kits (CWBIO, Beijing, China) following the manufacturer’s instructions. The 20 kb-gDNA library was prepared and sequenced on two SMRT cells with a PacBio Sequel system. Raw data were de novo assembled via SMARTdenovo (https://github.com/ruanjue/smartdenovo). Genome assembly completeness was assessed with both the Core Eukaryotic Genes Mapping Approach (CEGMA) [24 (link)] and Benchmarking Universal Single-Copy Orthologs (BUSCO; [25 (link)]). Protein-encoding genes were predicted with GLEAN [26 (link)] (http://sourceforge.net/projects/glean-gene) through both extrinsic and ab initio algorirhms.

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Wang X., Peng J., Sun L., Bonito G., Guo Y., Li Y, & Fu Y. (2020). Genome Sequencing of Paecilomyces Penicillatus Provides Insights into Its Phylogenetic Placement and Mycoparasitism Mechanisms on Morel Mushrooms. Pathogens, 9(10), 834.

Publication 2020

NuClean Plant Genomic DNA Kits

Cells Eukaryotic Gene Genome Library Mycelia Plant Protein genes Smrt

Corresponding Organization : Jilin Agricultural University

Other organizations : Shenyang Agricultural University, Michigan State University, Northeast Normal University

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Variable analysis

independent variables

Extraction of genomic DNA from mycelia using NuClean Plant Genomic DNA Kits

dependent variables

Genome assembly completeness assessed with CEGMA and BUSCO
Protein-encoding genes predicted with GLEAN

control variables

Manufacturer's instructions for NuClean Plant Genomic DNA Kits

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