Each fungal rice culture for the presence of mycotoxins was extracted and purified according to the method described by Urbaniak et al [37 (link)]. The liquid cultures from day 8 were subjected to mycotoxin quantification. The samples were collected from biological triplicates and were subjected to mycotoxin quantification.
Mycotoxin standards of high purity (fumonisins B1, B2, B3, enniatins A, A1, B, B1, beauvericin and moniliformin) were purchased from Sigma-Aldrich (Steinheim, Germany). Standard solutions of mycotoxins were prepared in methanol and kept in a freezer at −20 °C. LC/MS-grade organic solvents, water and other reagents were purchased from Sigma-Aldrich (Steinheim, Germany). Qualitative and quantitative analysis of mycotoxins were performed using a LC–PDA/TQD system consisting of an UPLC™ system (Acquity, Waters, Milford, MA, USA) coupled to a photodiode array detector (PDA) and a triple quadrupole mass spectrometer (TQD; Waters Micromass, Manchester, UK) according to the methods described in detail earlier [37 (link),49 (link)]
Mycotoxin standards of high purity (fumonisins B1, B2, B3, enniatins A, A1, B, B1, beauvericin and moniliformin) were purchased from Sigma-Aldrich (Steinheim, Germany). Standard solutions of mycotoxins were prepared in methanol and kept in a freezer at −20 °C. LC/MS-grade organic solvents, water and other reagents were purchased from Sigma-Aldrich (Steinheim, Germany). Qualitative and quantitative analysis of mycotoxins were performed using a LC–PDA/TQD system consisting of an UPLC™ system (Acquity, Waters, Milford, MA, USA) coupled to a photodiode array detector (PDA) and a triple quadrupole mass spectrometer (TQD; Waters Micromass, Manchester, UK) according to the methods described in detail earlier [37 (link),49 (link)]
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