Protein Extraction and Immunoblotting Assay

Proteins were extracted and visualized as reported in [34 (link)]. Separation of nuclear protein extracts from cytoplasmic proteins was performed by using a nuclear extraction kit (AbCam, Cambridge, UK) following manufacturer’s instructions. Immunoblotting was carried out with the following antibodies: anti-p70S6K(Thr389) #9206, anti-p70S6K #9202, anti-pAkt (Ser473) #4060, anti-Akt #9272, anti-S6(Ser235/236) ribosomal protein #2211, anti-S6 ribosomal protein 2217#, anti-4E-BP1(Thr37/46) #2855, anti-4E-BP1 #9644, anti-Acetyl-CoA carboxylase (ACC) #3662, anti- Stearoyl-CoA desaturase 1 (SCD1) #2794, and anti-Fatty Acid Synthase (FASN) #3189, provided by Cell Signalling Technology (Danvers, MA, USA). Anti-Erk #sc94, anti-Erk (Tyr204) #sc7383, anti-SREBP1 #sc-365513, anti-actin #sc-47778, and anti-lamin B #sc-374015 were obtained from Santa Cruz Biotechnology (Dallas, TX, USA).