Isolation and Expansion of Nasal and Intervertebral Disc Cells

Tissues were collected following local ethical committee approval (EKNZ-2015305, University Hospital Basel). After obtaining informed consent from all donors, human nasal septal cartilage tissue was harvested from the patients undergoing rhinoplasty (total n = 6, Supplmentary Table S1). NP tissue was acquired from donors undergoing surgery for DDD. Harvested NP tissues were graded using Pfirrmann scale (Urrutia et al., 2016 (link)). NP tissues with Pfirrmann grade 2–3 (mild/moderate degeneration) were used in this study (total n = 6, Supplementary Table S2). NC and NP cells were isolated after digestion in collagenase type II (0.15% for NC; 0.05% for NP cells for 22 h), and expanded in NC and NP expansion medium respectively (composition in Supplementary Material) up to passage 3.

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Kasamkattil J., Gryadunova A., Schmid R., Gay-Dujak M.H., Dasen B., Hilpert M., Pelttari K., Martin I., Schären S., Barbero A., Krupkova O, & Mehrkens A. (2023). Human 3D nucleus pulposus microtissue model to evaluate the potential of pre-conditioned nasal chondrocytes for the repair of degenerated intervertebral disc. Frontiers in Bioengineering and Biotechnology, 11, 1119009.

Publication 2023

Cartilage tissue Cells Collagenase Digestion Donors Human Nasal septal Patients Rhinoplasty Surgery Tissues

Corresponding Organization : University of Basel

Other organizations : Sechenov University

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Variable analysis

independent variables

Tissue source (nasal septal cartilage vs. nucleus pulposus)
Degree of disc degeneration in nucleus pulposus tissues (Pfirrmann grade 2-3)

dependent variables

Characteristics of isolated and expanded nasal cartilage (NC) and nucleus pulposus (NP) cells

control variables

Ethical committee approval (EKNZ-2015305, University Hospital Basel)
Informed consent from all donors
Collagenase type II digestion (0.15% for NC; 0.05% for NP cells for 22 h)
Cell expansion up to passage 3 in NC and NP expansion medium

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

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