Stripe Rust Resistance in Wheat-Thinopyrum Hybrid

TAI-14 was derived from the wheat–Th. intermedium partial amphiploid Zhong2 (He et al., 1989 ). It is a disomic addition line, containing a whole set of 42 wheat chromosomes and a pair of chromosomes derived from Th. intermedium (He et al., 1989 ; Han et al., 1998 ). We referred to the plant carrying two alien chromosomes as T14-44, the one carrying one alien chromosome as T14-43 and the one without an alien chromosome as T14-42, respectively. The wheat cultivar Jimai 22 was used as the recurrent parent to cross with the translocation line. Wheat cultivars Fielder and Jimai 20 were susceptible to stripe rust and used as parents to generate F₂ populations. Two highly stripe rust susceptible wheat varieties Mingxian 169 and Mianyang 11 were used as control in strip rust resistance evaluation. Th. intermedium accession PI 440001, T. urartu accession TMU38, and Aegilops tauschii accession TQ27 were used for the preparation of genomic DNA probes. T. asetivum line Chinese Spring (CS) and Ae. speltoides accession AE739 were used for the preparation of blocking DNA.