Fluorescence
spectra were
acquired using the Chirascan-plus qCD instrument (Applied Photophysics)
coupled to a Peltier system. The emission spectra (300–550
nm) were obtained by exciting a ∼10 μM protein sample
at 295 nm. The equilibrium thermal melt of the protein was recorded
from 278 to 368 K at 5 K intervals. Fluorescence lifetime measurements
were performed as described before.37 (link)
spectra were
acquired using the Chirascan-plus qCD instrument (Applied Photophysics)
coupled to a Peltier system. The emission spectra (300–550
nm) were obtained by exciting a ∼10 μM protein sample
at 295 nm. The equilibrium thermal melt of the protein was recorded
from 278 to 368 K at 5 K intervals. Fluorescence lifetime measurements
were performed as described before.37 (link)
