Following bisulfite treatment, the DNA methylation status of case and control subjects was assayed using the recently developed Infinium MethylationEPIC BeadChip microarray from Illumina, Inc. (San Diego, CA, USA) according to the manufacturer's instructions, which measured the methylation status of 853,307 CpG sites distributed over the whole genome. The image intensities were extracted using the Illumina iScan system (Illumina, Inc.) and quality-controlled using RnBeads (version 3.5) (18 (link)) in R (www.r-project.org/ ).
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Genome-wide DNA Methylation Profiling
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Corresponding Organization :
Other organizations : Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College
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Variable analysis
independent variables
- Bisulfite treatment
- DNA methylation status
- Case subjects
- Control subjects
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