After transfection for 48 h, the cells were seeded into 96-well plates at a density of 2 × 104 cell/well, incubated at 37°C containing 5% CO2 overnight, and supplemented with 10 μL CCK-8 solution in each well, followed by 4-h incubation at 37°C. Thereafter, the optical density value was read at 450 nm using an Elx800 Reader (Bio-Tek, Winooski, VT, USA) to assess the cell viability [18 (link)].
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