GCaMP6s and GCaMP6f plasmids were a gift from Douglas Kim (Addgene plasmid #40753 and #40755, respectively)19 (link). pET41a and pEGFP-N1 vectors were obtained from Novagen and Clontech, respectively. XL10-Gold and BL21 (DE3) Gold cells were purchased from Invitrogen. Restriction enzymes were obtained from New England Biolabs and T4 DNA ligase from Fermentas.
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