2′R-ochratoxin A (2′R-OTA) was synthetized as described [23 (link)]. Ochratoxin A (OTA), human serum albumin (HSA), bovine serum albumin (BSA), porcine serum albumin (PSA), rat serum albumin (RSA), racemic warfarin, furosemide, phenylbutazone, ibuprofen, methyl orange, bilirubin, and Dulbecco’s Modified Eagle Medium (DMEM) were obtained from Sigma-Aldrich (Saint Louis, MO, USA). Fetal bovine serum (FBS), (Pan-Biotech, Aidenbach, Germany), Bioluminescent ATP Assay Kit CLSII (Roche; Basel, Switzerland), and Coomassie Brilliant Blue G-250 (Reanal; Budapest, Hungary) were used as received. Stock solutions of 2′R-OTA and OTA (both 5000 µM) were prepared in ethanol (96 v/v%, Reanal, spectroscopic grade) and stored protected from light at −20 °C.
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