Subcutaneous Tumor Xenograft Model

LLC (5 x 10⁵) cells were inoculated subcutaneously in the right flank. The tumor volume was calculated using the formula V = lw² x 0.4 where l is the length and w the width [10 (link),11 (link),12 (link)]. Mice were monitored twice weekly by study participants at which time tumors were measured using calipers. Mice were euthanized prior to study endpoints if tumors were ulcerated or if the animals were moribund (obvious lack of grooming, extreme weight loss, signs of dehydration) or tumors were larger than 2500mm³. In this study, no animals were euthanized prior to study endpoints. Analgesic were not administered at any point during the study until euthanization. Blood was collected using EDTA-coated capillaries and analyzed with a Hemavet hematology analyzer (Drew Scientific). At sacrifice, blood was collected into 3.2% sodium citrate, centrifuged at 6000 rpm for plasma collection and centrifuged again at 12000 rpm to remove any contaminating cells. For brain collection, animals were perfused with 15ml of PBS under ketamine and xylazine anesthesia.

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Demers M., Suidan G.L., Andrews N., Martinod K., Cabral J.E, & Wagner D.D. (2018). Solid peripheral tumor leads to systemic inflammation, astrocyte activation and signs of behavioral despair in mice. PLoS ONE, 13(11), e0207241.

Publication 2018

Hemavet hematology analyzer

Analgesic Anesthesia Animals Blood Brain Capillaries Cells Dehydration Edta Ketamine Mice Plasma Sodium citrate Tumors Xylazine

Corresponding Organization : Boston Children's Hospital

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Variable analysis

independent variables

Inoculation of LLC (5 x 10^5) cells subcutaneously in the right flank of mice

dependent variables

Tumor volume calculated using the formula V = lw^2 x 0.4, where l is the length and w is the width
Hematological parameters measured using a Hemavet hematology analyzer
Brain collection after perfusion with 15 ml of PBS under ketamine and xylazine anesthesia

control variables

Twice-weekly monitoring of mice by study participants
Euthanasia of mice prior to study endpoints if tumors were ulcerated, animals were moribund, or tumors were larger than 2500 mm^3 (no animals were euthanized prior to study endpoints)
No administration of analgesics at any point during the study until euthanization
Blood collection using EDTA-coated capillaries and centrifugation for plasma collection

controls

No positive or negative controls were explicitly mentioned in the provided information.

Annotations

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