Lubrication Testing Materials Protocol

Reagents for lubrication testing materials were as described previously,^{2 (link),6 (link),33 (link)} including HA in a 4000-kDa form (Healon; Advanced Medical Optics). The antibody to PRG4 was anti-Lubricin from AbCam,^{2 (link)} nonspecific rabbit IgG was from Pierce, and mouse anti-rabbit IgG secondary antibody was from Jackson ImmunoResearch. Streptomyceshyaluronidase was from Seikagaku, SeaKem gold agarose was from Lonza; 50X TAE (2M Tris, 0.5 M ethylenediamine tetraacetic acid [EDTA]) electrophoresis buffer was from Life Technologies, Hybond-P polyvinylidene difluoride (PVDF) membrane for Western blotting was from GE Healthcare, and Stains-All was from Sigma-Aldrich.

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Grissom M.J., Temple-Wong M.M., Adams M.S., Tom M., Schumacher B.L., McIlwraith C.W., Goodrich L.R., Chu C.R, & Sah R.L. (2014). Synovial Fluid Lubricant Properties Are Transiently Deficient After Arthroscopic Articular Cartilage Defect Repair With Platelet-Enriched Fibrin Alone and With Mesenchymal Stem Cells. Orthopaedic Journal of Sports Medicine, 2(7), 2325967114542580.

Publication 2014

anti-Lubricin mouse anti-rabbit IgG secondary antibody Streptomyceshyaluronidase SeaKem gold agarose Hybond-P polyvinylidene difluoride (PVDF) membrane Stains-All

Agarose Anti igg Antibody Buffer Electrophoresis Ethylenediamine tetraacetic acid Gold Healon Lubrication Lubricin Membrane Mouse Optics Polyvinylidene difluoride Rabbit Stains all Tris

Corresponding Organization :

Other organizations : University of California, San Diego, Colorado State University, Stanford University

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Variable analysis

independent variables

HA in a 4000-kDa form (Healon; Advanced Medical Optics)
Anti-Lubricin from AbCam
Nonspecific rabbit IgG from Pierce
Mouse anti-rabbit IgG secondary antibody from Jackson ImmunoResearch
Streptomyces hyaluronidase from Seikagaku
SeaKem gold agarose from Lonza
50X TAE (2M Tris, 0.5 M ethylenediamine tetraacetic acid [EDTA]) electrophoresis buffer from Life Technologies
Hybond-P polyvinylidene difluoride (PVDF) membrane for Western blotting from GE Healthcare
Stains-All from Sigma-Aldrich

dependent variables

Lubrication testing materials

control variables

Reagents for lubrication testing materials as described previously

positive controls

None specified

negative controls

Nonspecific rabbit IgG from Pierce

Annotations

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