On the first day, the rats were sensitized by injecting 0.6 ml of (0.9% NaCl) normal saline comprising alum (4 mg) and ovalbumin (2 mg) into four-foot pads. They were boosted five days later with a subcutaneous injection of 1 ml of normal saline comprising ovalbumin (2 mg) at ten locations on the back. Later, from day 8 to 15 d, local sensitization was performed by instilling ovalbumin in normal saline (30 µl) per site into bilateral eyes with a micropipette, and the symptoms were assessed.
Protocol detail
Ovalbumin-Induced Rat Allergy Model
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Other organizations : Manipal Academy of Higher Education
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Variable analysis
independent variables
- Injection of 0.6 ml of (0.9% NaCl) normal saline comprising alum (4 mg) and ovalbumin (2 mg) into four-foot pads on the first day
- Boosting with a subcutaneous injection of 1 ml of normal saline comprising ovalbumin (2 mg) at ten locations on the back, five days later
- Instilling ovalbumin in normal saline (30 µl) per site into bilateral eyes with a micropipette, from day 8 to 15
- Symptoms assessed
- Kept constant to ensure valid results
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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