Human genomic DNA samples were derived from anonymous Polish unrelated volunteers who declared to be healthy. Samples were randomly selected from the “normal Polish population” genetic collection at the Biobank Lab, Department of Molecular Biophysics, University of Lodz. Genetic material for this collection was sampled in 2011 – 2012 within the EU-funded TESTOPLEK project. All subjects gave their written informed consent to participate in the study. This study was approved by the relevant regional ethical committee (Research Bioethics Commission, University of Lodz - Decision no. 8/KBBN-UŁ/II/2014 and Statement of the Research Bioethics Commission, University of Lodz from 17th of June, 2010) and all procedures were performed in accordance with the Declaration of Helsinki.
Saliva was collected into Oragene OG-500 DNA collection/storage receptacles (DNA Genotek, Kanata, Canada) and genomic DNA was subsequently isolated by the MagNA Pure LC DNA Isolation Kit - Large Volume (Roche, Basel, Switzerland) with final concentration normalized to 200 pg/μl [27 (link)]. A total of 190 samples were enrolled in the scanning study and other 380 in genotyping.
Saliva was collected into Oragene OG-500 DNA collection/storage receptacles (DNA Genotek, Kanata, Canada) and genomic DNA was subsequently isolated by the MagNA Pure LC DNA Isolation Kit - Large Volume (Roche, Basel, Switzerland) with final concentration normalized to 200 pg/μl [27 (link)]. A total of 190 samples were enrolled in the scanning study and other 380 in genotyping.
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