Genetic Modifier Screening in Drosophila

All stocks used for our modifier screen were obtained from the Bloomington Drosophila Stock Center (Indiana, USA) and are listed in Results. The GMR-GAL4; UAS-cindr^RNAi2.21A/ SM5: TM6b line was generated from UAS-cindr^RNAi2.21A transgene [13 (link)] and the GMR-GAL4 driver line [17 (link)]. In addition, we utilized the following stocks: Canton-S, w¹¹¹⁸, UAS-lacZ and UAS-puc (gifts from R. Cagan), and bsk¹ (Bloomington stock number BL-3088), UAS-bsk (BL-9310), cbl^F165 (BL-9676), nopo^excl42 (BL-57335), nopo^Z1447 (BL-57334), puc^H246 (BL-4390), UAS-slpr^WT-HA (BL-58820), Traf4^EY09771 (BL-17600), UAS-Traf6.S (BL-58991) and Uev1a^DG14805 (BL-20440).

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Ketosugbo K.F., Bushnell H.L, & Johnson R.I. (2017). A screen for E3 ubiquitination ligases that genetically interact with the adaptor protein Cindr during Drosophila eye patterning. PLoS ONE, 12(11), e0187571.

Publication 2017

Canton-S w1118 UAS-lacZ UAS-puc

Drosophila Gifts Lacz Traf6 Transgene

Corresponding Organization : Wesleyan University

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Protocol cited in 3 other protocols

Variable analysis

independent variables

Genotypes/Transgenes
RNAi knockdown of cindr

dependent variables

Not explicitly mentioned

control variables

Canton-S
W^1118
UAS-lacZ
UAS-puc
Bsk^1
UAS-bsk
Cbl^F165
Nopo^excl42
Nopo^Z1447
Puc^H246
UAS-slpr^WT-HA
Traf4^EY09771
UAS-Traf6.S
Uev1a^DG14805

positive controls

UAS-puc

negative controls

Canton-S
W^1118
UAS-lacZ

Annotations

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