Single-cell suspensions were prepared from corneas as previously described.11 (link) In brief, corneas were digested in RPMI media (Lonza, Walkersville, MD, USA) containing 2 mg/mL collagenase type IV (Sigma-Aldrich Corp., St. Louis, MO, USA) and 2 mg/mL DNase I (Roche, Basel, Switzerland) for 45 minutes at 37°C and then filtered through a 70-μm cell strainer.
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