Genome-wide miRNA Expression Analysis

miRNA microarray was conducted as described previously [68 (link)]. miRNAs were extracted using TRIzol^®. RNA quantity and quality were determined using a NanoDrop^™ 2000 spectrophotomer (Thermo Scientific). Affymetrix GeneChip^® miRNA 2.0 arrays were used for genome-wide miRNA-expression analysis (15,644 probe sets for 131 organisms, including 1105 mature human miRNAs, 1105 human pre-miRNAs, 722 mature mouse miRNAs and 690 mouse pre-miRNAs) according to the instruction manual (Affymetrix, Santa Clara). Two biological repeats were used for each treatment.

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Menachem A., Makovski V., Bodner O., Pasmanik-Chor M., Stein R., Shomron N, & Kloog Y. (2016). Intercellular transfer of small RNAs from astrocytes to lung tumor cells induces resistance to chemotherapy. Oncotarget, 7(11), 12489-12504.

Publication 2016

GeneChip

Biological Genechip Genome Human Microarray Mirnas Mouse Pre mirnas Trizol

Corresponding Organization : Tel Aviv University

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Variable analysis

independent variables

Treatment

dependent variables

MiRNA expression

control variables

RNA quantity and quality determined using NanoDrop™ 2000 spectrophotomer (Thermo Scientific)
Affymetrix GeneChip® miRNA 2.0 arrays used for genome-wide miRNA-expression analysis

controls

Two biological repeats for each treatment

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