RNA extraction and cDNA synthesis was performed as previously described15 (link)–17 (link),23 (link). B. malayi adult worms were snap frozen and crushed into fine powder in a 1.5 mL micro-centrifuge tube using Kimble™ Kontes™ Pellet Pestle™ (Fisher Scientific, USA). Total RNA was extracted using TRIzol® Reagent (Life Technologies, USA) according to the manufacturer’s instructions. About 1 µg of total RNA was used to synthesize cDNA using SuperScript® VILO™ Master Mix (Life Technologies, USA). Samples were either used to amplify DNA using PCR or stored at − 20 °C for later use.
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