Transient Expression of PvTET8-1 in Nicotiana benthamiana

The 35S:PvTET8-1-GFP construct was electroporated into A. tumefaciens strain CV3010. Preparing A. tumefaciens (OD₆₀₀ 0.5) for infiltration into N. benthamiana epidermal cells were done on leaves from 4- to 6-week-old wild type plants as previously described (Norkunas et al., 2018 (link)). Leaves transformed with the vector harboring the 35S::GFP was used as the control. Images of transiently infected leaves were observed using confocal laser scanning microscopy (Nikon Eclipse Ti-E) after 72 h of infiltration. The subcellular localization of PvTET8-1 was determined in P. vulgaris root hairs from 4-day-old transgenic hairy roots, generated with A. rhizogenes carrying 35S::PvTET8-1-GFP. Root hairs were observed using confocal laser scanning microscopy (Nikon Eclipse Ti-E).