Characterization of GBM and Fetal Brain

Characteristics of 8 GBMs (GBM146, 157, 205, 206, 218, 1600, 2313, and 13) and 2 non-tumor human fetal brain specimens (f16w and 1105A) from aborted fetus were published previously (15 (link)–18 (link)). Methods for establishment of GBM sphere cultures and normal sphere cultures are published previously (16 (link), 18 (link)) and details are described in Supplementary Information. All the experiments were carried out with short-term cultures within 20 passages.

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Miyazaki T., Pan Y., Joshi K., Purohit D., Hu B., Demir H., Mazumder S., Okabe S., Yamori T., Viapiano M., Shin-ya K., Seimiya H, & Nakano I. (2012). Telomestatin Impairs Glioma Stem Cell Survival and Growth through the Disruption of Telomeric G-Quadruplex and Inhibition of the Proto-oncogene, c-Myb. Clinical cancer research : an official journal of the American Association for Cancer Research, 18(5), 1268-1280.

Publication 2012

Aborted fetus Brain Fetal Human Tumor

Corresponding Organization : The Ohio State University Comprehensive Cancer Center – Arthur G. James Cancer Hospital and Richard J. Solove Research Institute

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Protocol cited in 6 other protocols

Variable analysis

independent variables

Establishment of GBM sphere cultures
Establishment of normal sphere cultures

dependent variables

Characteristics of 8 GBMs (GBM146, 157, 205, 206, 218, 1600, 2313, and 13)
Characteristics of 2 non-tumor human fetal brain specimens (f16w and 1105A)

control variables

Short-term cultures within 20 passages

controls

Positive controls not explicitly mentioned
Negative controls not explicitly mentioned

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