Rat Aortic Smooth Muscle Cell Culture

Rat aortic smooth muscle cells (RASMCs; Lonza) were cultured as previously described (Rahaman et al., 2017 (link)). In brief, RASMCs were cultured at 37°C in Lonza Smooth Muscle Growth Medium-2 with SmGm-2 SingleQuot kit (Lonza) supplementation. Cells were passaged using Gibco Trypsin/EDTA up to passage 12. For treatment, FoxO inhibitor AS1842856 (Cayman) was dissolved in DMSO at 10 mM stock concentration and diluted in DMSO for further experiments. Control and treatment DMSO concentration was 0.1% of the total volume for treatment periods.

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Galley J.C., Durgin B.G., Miller M.P., Hahn S.A., Yuan S., Wood K.C, & Straub A.C. (2019). Antagonism of Forkhead Box Subclass O Transcription Factors Elicits Loss of Soluble Guanylyl Cyclase Expression. Molecular Pharmacology, 95(6), 629-637.

Publication 2019

RASMCs Smooth Muscle Growth Medium-2 SmGm-2 SingleQuot kit

Aortic As1842856 Cayman Cells Cultured medium Dmso Edta Smooth muscle Smooth muscle cells Trypsin

Corresponding Organization :

Other organizations : University of Pittsburgh

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Variable analysis

independent variables

FoxO inhibitor AS1842856 (Cayman) concentration

dependent variables

Not explicitly mentioned

control variables

Rat aortic smooth muscle cells (RASMCs; Lonza)
Lonza Smooth Muscle Growth Medium-2 with SmGm-2 SingleQuot kit (Lonza) supplementation
Cell passage up to passage 12
DMSO concentration (0.1% of the total volume)

controls

Control (0.1% DMSO in the total volume)

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