IC50 of Silmitarsertib on SW480 wild-type and SW480 SALL2 KO cells were determined by crystal violet Assay. Five thousand cells were seeded on 96-well plates and incubated with increasing concentrations of Silmitarsertib (0–40 μM) for 24 h at 37 °C. Cells were stained with 0,01% (p/v) crystal violet [19 (link)] and fluorescence (680 nm) was quantified using LI-COR ODYSSEY CLX equipment.
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